Kaitlyn Freeman

Document Type


Publication Date



The moss Physcomitrum patens is an attractive model organism, due to its small genome and dominant haploid phase. P. patens has eight genes that form a family known as the Cellulose Synthase-Like Ds (CSLDs). A previous study using global knockdown of the entire PpCSLD family by RNA interference (RNAi) showed a decrease in protonemal tip growth, indicating that PpCSLDs play a role in protonemal growth. In order to study the roles of specific CSLDs in P. patens development, triple knockout mutants for PpCSLD3/4/5 were generated by CRISPR/Cas9 mutagenesis. Plant cell walls help plants deal with osmotic stress. Therefore a salt tolerance assay was completed in order to phenotype the ppcsld3/4/5 KO mutants. Wild-type P.patens and ppcsld3/4/5 KO mutants were exposed to high salinity at varying concentrations (150 mM, 250 mM, 275 mM, 300 mM 325 mM, and 350 mM NaCl) and imaged. Colorimetric analysis of the images showed that ppcsld3/4/5 KO mutants had significantly less (p < 0.001) percent green, when comparing the combined means across all treatments.

Faculty Mentor

Christos Dimos, Ph.D.

Academic Discipline

College of Arts & Sciences



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